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Workshops

Interactions and Transport of Charged Species in Bulk and at Interfaces

July 4, 2016 to July 7, 2016
Location : CECAM-AT

Phase Behavior of Lysozyme Dispersions

Christoph Goegelein


Abstract

Understanding the phase behavior of charged colloidal dispersions is of great interest for food products, oil- and water-based paints, and biotechnological applications. In the latter case, for example, the challenge is to predict solution conditions that enhance protein nucleation and crystal growth. For this purpose, models are needed that describe protein-protein interactions accurately. We introduce a DLVO-like model that describes the effect of additives such as dimethyl sulfoxide and glycerol on the phase diagram of lysozyme. In addition, we present a simple model that takes into account the influence of anisotropic attractions on the phase behavior of lysozyme solutions. The quantitative agreement between the experimental and theoretically predicted coexistence curves indicates that anisotropic interactions are crucial to understand the phase behavior of protein solutions. Moreover, the simple model gives additional evidence that the anisotropic attractions are caused
by hydrophobic patches on the lysozyme surface.



References

[1] C. Gögelein, G. Nägele, R. Tuinier, T. Gibaud, A. Stradner, and P. Schurtenberger, J. Chem. Phys. 129, 085102 (2008)

[2] C. Gögelein, D. Wagner, F. Cardinaux, G. Nägele, and S. U. Egelhaaf, J. Chem. Phys. 136, 015102 (2012)

[3] C. Gögelein, F. Romano, F. Sciortino, and A. Giacometti, J. Chem. Phys. 136, 094512 (2012)

[4] A. Giacometti, C. Gögelein, F. Lado, F. Sciortino, S. Ferrari, and G. Pastore, J. Chem. Phys. 140, 094104 (2014)